Cell Line Mycoplasma
Diagnostics provides a PCR (Polymerase Chain Reaction) screening
service to detect any mycoplasma contamination that may be present in
your cell lines.
Microbiological Methods 39 121-126
of the genus ‘Mycoplasma’ are the smallest organisms without
cell walls capable of self-replication. They can cause various different
diseases in humans, animals, insects, and plants. In cell culture lines
mycoplasma contamination can be a serious issue because of its potential
to cause adverse effects on cellular metabolism.
by Mycoplasmas and Acholeplasmas (Mollicutes) is one of the most frequently
occurring problems associated with cell cultures and by affecting many
different biological aspects of the infected culture, leads to unreliable
experimental results and potentially harmful biological products derived
from the cells.
testing for Mycoplasma and Acholeplasma contamination is therefore essential
to ensure reliable research results.
surveys have shown that 15 to 80% of cell cultures tested were contaminated
with mollicutes, but typically this is not always easily detected as
it produces neither turbidity in the culture (an easily observed effect
of bacterial contamination) or the cytopathic effects often seen with
viral contamination 1.
Diagnostics can screen for all species of cell culture contaminating
used by Surrey Diagnostics yield an extremely high
level of sensitivity and accuracy, giving you consistently high quality
method of confirmation is also available through our capability to culture
samples within our own laboratories, should you require it.
J. Tang, M. Hu, S. Lee, R.Roblin (2000)
A polymerase chain reaction based method for detecting Mycoplasma /
Acholeplasms contaminants in cell culture.