Diagnostics provides a range of genetic modification screening
services for your transgenic animal colonies. All of our screening services
are confidential and tailored to your individual needs.
of transgenic animals has increased markedly during recent years. This
means that it is necessary to screen breeding colonies in order to establish
which progeny carry the appropriate genetic modification.
facilities have found that this is difficult to organise in-house and
there are often large numbers of untested mice in the facility, taking
up valuable housing space. This is particularly important now that Full
Economic Costing has been introduced. Surrey Diagnostics
can screen your animals for you, enabling you to keep only
the animals that have the correct genetic modification.
for Surrey Diagnostics to establish a PCR test for
your requirements we will require the following:
Primer sequences or your prepared primers
• Test sample that is homozygous positive for the
• Test sample that is heterozygous for the modification
• A basic SOP to follow
When the test has been validated your samples can be analysed at a rate
of nearly 100 samples per day for simple one-step PCR tests. The test
sample can be a tail clip or possibly a sample of hair bulbs 1.
Diagnostics are able to screen your animal colonies for
the ob gene. The test sample can be a tail clip, an ear clip or hair
bulb samples. Testing in this way eliminates the need to retain animals
until the phenotype is evident. By using PCR testing, you are able to
identify immediately those which are ob/ob, ob/+ or +/+.
Diagnostics can screen any mammalian species to determine gender
through the sex chromosomes. The test sample can be blood, tissue or
hair bulb samples.
for the xx/yy genes allows you to identify the gender of undeveloped
foetuses or any animals with abnormal development.
1 DNA detection
in hair of transgenic mice - a simple technique minimising the distress
on the animals, Laboratory Animals (1999) 33,385-398.